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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Breeding by Design for Functional Rice with Genome Editing Technologies
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A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI-Edit via LbCas12a Activity Optimization.

Dawei Liang1,2, Huanhuan Guo2, Juan Wei2

  • 1State Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, China.

Plant Biotechnology Journal
|July 4, 2026
PubMed
Summary

Researchers boosted maize genome editing efficiency using haploid induction (HI-Edit) by optimizing CRISPR-Cas protein delivery and applying heat treatment. This enhances the yield of edited doubled haploid (DH) plants, offering a scalable framework for crop improvement.

Keywords:
UBA2 fusiongenome editinghaploid editing rate (HER)haploid induction (HI)zygote editing rate (ZER)

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Genetics

Background:

  • Haploid induction coupled with genome editing (HI-Edit) allows direct modification of elite crop varieties.
  • Low haploid editing rates (HER) limit the widespread application of HI-Edit.
  • Improving HER is crucial for efficient genome editing in crops.

Purpose of the Study:

  • To enhance maize HI-Edit efficiency.
  • To overcome bottlenecks in haploid editing rates.
  • To develop a scalable framework for elite-line genome editing.

Main Methods:

  • Optimized LbCas12a variant (LbCas12aV) expression in sperm cells and zygotes.
  • Applied post-pollination heat treatment.
  • Fused LbCas12aV with the UBA2 domain to improve protein stability.

Main Results:

  • Heat treatment alone increased HER to 19.1%, a 12-fold improvement.
  • UBA2 fusion improved HER by 6-fold at the Waxy1 locus and 4.5-fold at the Glossy2 locus.
  • Combined UBA2 fusion and heat treatment achieved an average HER of 25%, with a maximum of 33%.

Conclusions:

  • Increasing CRISPR-Cas protein abundance and modulating environmental conditions significantly improve HI-Edit efficiency.
  • The developed framework is robust, scalable, and transferable to other crops.
  • This advancement facilitates efficient genome editing in elite crop lines.