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Related Experiment Video

Updated: Jul 5, 2026

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Evaluating a Novel Cell-Free Preservation Solution for Human Cardiomyocyte Protection: A Proof-of-Concept Study.

Christian Beckers¹, Sandra Kraemer², Alexander Theißen^1,3

¹Department of Anaesthesiology, Medical Faculty, University Hospital RWTH Aachen, Aachen, Germany, ukaachen.de.

Biomed Research International

|July 4, 2026

Summary

A new cell-free preservation solution, Omnisol, shows promise for maintaining human cardiomyocyte function under cold and warm storage. Omnisol outperformed Custodiol in preserving metabolic activity and reducing apoptosis during normothermic preservation.

Keywords:

cardiomyocytes cell free normothermic organ preservation

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Related Experiment Videos

Last Updated: Jul 5, 2026

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Published on: June 22, 2020

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Published on: December 16, 2022

Area of Science:

Cardiology
Regenerative Medicine
Biomedical Engineering

Background:

Heart transplantation is limited by donor availability and preservation injury.
Static cold storage (SCS) with Custodiol is standard but increases primary graft dysfunction risk.
Advanced preservation solutions are needed for both cold and normothermic conditions.

Purpose of the Study:

Compare Omnisol and Custodiol for preserving human cardiomyocyte function.
Assess effects on contractile function, metabolic activity, and apoptosis.
Evaluate performance under static cold and normothermic storage.

Main Methods:

Human-induced pluripotent stem cell-derived cardiomyocytes were stored in medium, Custodiol, or Omnisol.
Storage conditions included static cold and normothermic preservation.
Functional assessment involved motion analysis, MTT assay, and TUNEL staining.

Main Results:

Omnisol preserved normothermic cardiomyocyte contractility comparable to standard medium.
Custodiol showed reduced beating frequency and impaired recovery.
Omnisol maintained higher metabolic activity and lower apoptosis under normothermic conditions.

Conclusions:

Omnisol demonstrates potential for preserving human cardiomyocytes in cold and normothermic conditions.
Findings support Omnisol's evaluation for dynamic organ perfusion strategies.
This study is a proof-of-concept for a novel cell-free preservation solution.