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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Antisense Oligonucleotide-Mediated Chimeric RNA Knockdown
Anam Tajammal1, Katie Do1, Hui Li2,3
1Department of Pathology, University of Virginia, Charlottesville, VA, USA.
Abstract:
Knockdown assays aim to silence the expression of a target gene. By reducing expression of a gene, researchers can investigate the gene's function in biological processes. Common knockdown assays use RNA interference (RNAi) to reduce gene expression by degrading its mRNA. However, RNAi requires the use of double-stranded RNA to target mature mRNA transcripts. Antisense oligonucleotides (ASO) offer an alternative pathway to knocking down gene expression by allowing the use of single-stranded DNA or RNA to target pre-mRNA, mature mRNA, long non-coding RNAs or DNA. Here, we have provided guidelines and procedures for ASO knockdown, application to chimeric RNAs, and necessary controls for a successful experiment.
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