Construction of a Cyclic Peptide Library for Phage Display

Phage display is a powerful high-throughput screening technique used to screen diverse peptide libraries against therapeutically relevant protein targets. Compared to linear peptides, cyclic peptides have improved stability and binding characteristics. There are various methods to construct cyclic peptide phage display libraries, which can be screened against targets to identify cyclic peptide candidates. These peptides can then be optimized to enhance potency and specificity. In this chapter, we outline a strategy to generate cyclic peptide phage display libraries using a bischlooroxime crosslinker.