Related Experiment Video
Updated: Jul 6, 2026

07:35
Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
Published on: June 14, 2021
A Simple and Rapid Method for Preparing VHHAntibody-Template DNA Conjugates for Loop-Mediated Isothermal
Junpei Yamashita1, Naoto Nemoto2
1Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2026
Abstract:
Generally, in vitro display technology serves as a fundamental method for performing in vitro selection by linking genotypes to phenotypes. This study introduces a rapid and simple method for preparing VHH antibody-template DNA conjugate suitable for the LAMP method (Loop-mediated isothermal amplification), known for its rapid and highly sensitive detection, using cDNA display, an in vitro display technology.
Keywords:
Antibody-DNA conjugateImmune PCRIsothermal PCRLoop-mediated isothermal amplification (LAMP)VHHcDNA display
