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Related Experiment Video

Updated: Jul 6, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

Application of Phage-Displayed Peptide Library for Epitope Mapping.

Belle Li Ling1, Angela Chiew Wen Ch'ng1, Yee Siew Choong1

  • 1Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia.

Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2026
PubMed
Summary

Identifying antibody epitopes is crucial for understanding immune responses and developing therapies. Peptide phage display offers a rapid method for mapping these critical antigen-binding sites on monoclonal antibodies.

Keywords:
BiopanningEpitope mappingMonoclonal antibodiesPeptide libraryPhage display

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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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Last Updated: Jul 6, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Antibodies are key components of the adaptive immune system, responsible for recognizing specific antigens.
  • The interaction between an antibody's paratope and an antigen's epitope dictates immune specificity.
  • Accurate epitope identification is vital for numerous biomedical applications, including diagnostics and therapeutics.

Purpose of the Study:

  • To outline a protocol for identifying antibody epitopes using peptide phage display.
  • To demonstrate the utility of peptide phage display for epitope mapping of monoclonal antibodies.

Main Methods:

  • Utilized a peptide phage display library for high-throughput screening.
  • Applied the method to identify the epitope of a specific recombinant monoclonal antibody.

Main Results:

  • Successfully identified the epitope for the target monoclonal antibody.
  • Demonstrated the efficiency and speed of peptide phage display for this purpose.

Conclusions:

  • Peptide phage display is an effective and rapid technique for epitope identification.
  • This method provides valuable insights into antibody-antigen interactions for biomedical research.