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Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
Published on: January 26, 2016
De Novo Discovery of Prenylated Macrocyclic Peptide Ligands
Sota Uchida1,2, Hiroaki Suga3, Yuki Goto4,5,6
1Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto, Japan.
Abstract:
Prenylated peptides, endowed with hydrophobic prenyl groups, are widely found as secondary metabolites in nature. To develop de novo artificial prenylated peptide ligands with selective affinity for a target protein of choice, a random prenylated peptide library can be constructed through the combination of a custom-made in vitro translation system and a peptide prenyltransferase, which is subsequently applied to mRNA display screening. In this chapter, we describe protocols for constructing a prenylated thioether-closed macrocyclic peptide library and conducting mRNA display screening to identify selective ligands.
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