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A chemiluminescence sensor for ciprofloxacin detection based on copper ion and aptamer co-modified magnetic
Jin Ling1, Jiangle Yi1, Yuanling Sun1
1Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Abstract:
In this paper, magnetic microspheres (MS) co-modified by copper ion (Cu2+) and aptamer (Apt) were prepared and used to construct a chemiluminescence (CL) sensor for the detection of ciprofloxacin (CIP) by combining the magnetic functional materials with the flow injection-chemiluminescence (FI-CL) instrument. When CIP is present in the sample, the Apt on the surface of MS- Apt-Cu2+ will recognize and bind with CIP, resulting in the release of Cu2+ from the surface of MS-Apt-Cu2+ into the supernatant after magnetic separation. Subsequently, the quantitative detection of CIP is achieved through a FI-CL instrument. Under optimal conditions, the constructed sensor for the detection of CIP exhibited a wide linear range of 0.001-500 ng/mL and a low detection limit of 0.36 pg/mL. The sensor also has remarkable selectivity, reproducibility, and stability, and was successfully applied to CIP determination in lake water and milk samples, demonstrating its potential for monitoring antibiotic residues in environmental and food samples.
