Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing tumor burden-based classification with traditional methods for knowledge-based VMAT planning in multiple brain metastases.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026
Same author

Spatial collimation sequencing enhances low-dose sparing and geometric robustness in single-isocenter SBRT for multiple liver metastases.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026
Same author

Positioning uncertainties in single-target longitudinal segmentation for hippocampal-avoidance whole brain radiotherapy using volumetric modulated arc therapy.

Physics and imaging in radiation oncology·2025
Same author

Integrated Transcriptomic and Metabolomic Analysis Reveals Biochar-Induced Enhancement of Growth and Secondary Metabolism in the Medicinal Plant <i>Echinacea purpurea</i>.

International journal of molecular sciences·2025
Same author

Mussel Foot-Inspired Multifunctional Engineered Protein Adhesive with Enhanced Antibacterial Properties.

Biomacromolecules·2025
Same author

The impact of environmental factors on respiratory tract microbiome and respiratory system diseases.

European journal of medical research·2025

Related Experiment Video

Updated: Jul 6, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

Shear force-induced gene expression system achieved through mechanically responsive LacI.

Yue Xiao1, Liuyanlin Zhang1, Yawen Fu1

  • 1Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, MOE Key Laboratory of Molecular Biophysics, Wuhan, 430074, China.

International Journal of Biological Macromolecules
|July 4, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel, green gene expression system using mechanical force for induction. The system achieves significant protein expression without chemical inducers, offering a sustainable alternative.

Keywords:
LacIMechanical regulationShear force

More Related Videos

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
08:50

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

Published on: October 21, 2018

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
08:50

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

Published on: October 21, 2018

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemical Engineering

Background:

  • Induced expression systems are crucial for regulating proteins and biochemical processes.
  • Shear force induction offers a simple, pollution-free, and sustainable method for biological process control.

Purpose of the Study:

  • To develop a novel mechanically inducible gene expression system.
  • To utilize peptide sequences as mechanical response elements fused with LacI.

Main Methods:

  • Designed and produced three mechanical response LacI constructs using (VPGEG)n sequences (n=9, 18, 36).
  • Verified the expression, structure, and function of the constructs.
  • Optimized parameters, including shaking speeds, to enhance induction efficiency.

Main Results:

  • Successfully developed a novel mechanically inducible expression system.
  • Demonstrated that induction effect correlates with shaking speed intensity.
  • Achieved a 6.8-fold induction effect without chemical inducers.
  • Showcased applicability for expressing fluorescent and functional proteins with enzymatic activity.

Conclusions:

  • A new non-invasive mechanical induction method for gene expression regulation has been established.
  • The developed system provides a sustainable and efficient alternative to traditional chemical induction methods.