Shear force-induced gene expression system achieved through mechanically responsive LacI

Induced expression systems have been widely used in the regulation of proteins and biochemical processes. Shear force induction, thanks to its simplicity and pollution-free nature, offers unique advantages for green and sustainable processes. In this study, (VPGEG)₉, (VPGEG)₁₈, and (VPGEG)₃₆ sequences were utilized as mechanical response tools, fused with the LacI sequence, and three mechanical response LacI constructs were designed and produced. After verifying their expression, structure, and function, we successfully developed a novel mechanically inducible expression system. The induction effect of the system is closely related to the intensity of the shaking speeds. After parameter optimization, the induction effect reached 6.8-fold, and no additional chemical inducers were required. This system exhibits excellent applicability, as it can be used for the expression of fluorescent proteins and effectively induces the expression of functional proteins with good enzymatic activity. This study provides a new non-invasive mechanical induction method for gene expression regulation.