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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Programmable DNA probe-mediated nanopore biosensor for multiplex nucleic acid detection and its application in milk
Jianghua Liu1, Yuxuan Meng1, Jianing Chen1
1School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Analytica Chimica Acta
|July 4, 2026
Summary
This study presents a novel nanopore biosensing strategy for sensitive, label-free detection of multiple nucleic acid targets. The method uses click chemistry and host-guest complexes for high-discrimination signal output, applicable to complex food matrices.
Area of Science:
- Nanopore biosensing
- Nucleic acid detection
- Click chemistry
Background:
- Multiplex nucleic acid detection in complex matrices is challenging.
- Existing methods may lack sensitivity or specificity.
Purpose of the Study:
- To develop a novel nanopore biosensing strategy for simultaneous, label-free, and high-discrimination detection of multiple nucleic acid targets.
- To demonstrate the platform's applicability in complex food matrices.
Main Methods:
- Integration of programmable DNA probes with click chemistry amplification.
- Utilizing host-guest complexes (cucurbit[6]uril) as signal tags.
- Employing α-hemolysin (α-HL) nanopore for signal analysis and target-specific asymmetric PCR-triggered strand displacement for probe release.
Main Results:
- Achieved label-free, high-discrimination detection of multiplex nucleic acid targets.
- Demonstrated a satisfactory limit of detection (LOD) as low as 18.78 pM.
- Successfully applied the method to detect adulterated DNA in goat milk samples, showcasing applicability in complex food matrices.
Conclusions:
- The developed nanopore biosensing strategy offers a sensitive and specific platform for multiplex nucleic acid detection.
- The strategy shows promise as a universal platform for detecting various nucleic acid markers in diverse matrices, including food authenticity testing.
