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Updated: Jul 6, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Metastable multimeric G-quadruplex 2'FY-RNA aptamers that selectively bind pyoverdines.

Sharif Anisuzzaman^1,2,3, Marit Nilsen-Hamilton^4,5,6

¹Ames Laboratory, U. S. Department of Energy, Ames, IA, USA. sharif@aptalogic.com.

Scientific Reports

|July 4, 2026

Summary

This summary is machine-generated.

Novel RNA aptamers bind pyoverdine-Pf5 (PVD-Pf5) and form G-quadruplex structures. These multimeric G-quadruplexes offer potential for RNA-based regulatory mechanisms.

Keywords:

2′FY-RNA Aptamer G-quadruplex Metastable Pyoverdine Pf5

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Area of Science:

Biochemistry
Molecular Biology
RNA Aptamer Technology

Background:

Pyoverdines are siderophores with a peptide component.
RNA aptamers can be selected for specific molecular targets.
G-quadruplexes are non-canonical nucleic acid structures with regulatory potential.

Purpose of the Study:

To select and characterize novel 2'FY-RNA aptamers that bind pyoverdine-Pf5 (PVD-Pf5).
To investigate the structural properties and formation of G-quadruplexes by these aptamers.
To explore the potential of multimeric G-quadruplexes in RNA-based regulation.

Main Methods:

Systematic Evolution of Ligands by Exponential Enrichment (SELEX) for aptamer selection.
Spectroscopic techniques (fluorescence, CD, NMR) for structural and binding analysis.
Computational modeling and electrophoretic methods for structural elucidation.

Main Results:

Two distinct 2'FY-RNA aptamers were identified with specific binding to PVD-Pf5, showing fluorescence enhancement.
Aptamers recognized the peptide portion of pyoverdines and formed thermally metastable G-quadruplex structures.
Dimeric G-quadruplexes were formed, exhibiting pressure stability and requiring cations for PVD-Pf5 binding but not for G-quadruplex formation.

Conclusions:

Selected aptamers represent novel sequences expanding the repertoire of multimeric G-quadruplexes.
These aptamers and their G-quadruplex structures provide a framework for structure-based regulatory mechanisms in cellular RNAs.
The findings highlight the potential of G-quadruplexes as versatile functional motifs in RNA biology.