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Updated: Jul 6, 2026
12:23
Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
Published on: August 2, 2018
Advanced (Nano)material-Based Enrichment Strategies for Quantitative Phosphoproteomics Analysis by Mass Spectrometry
Inmaculada Ortiz-Gómez1, Francisco Calderón Celis2
1Centro Tecnológico de Componentes-CTC, Scientific and Technological Park of Cantabria (PCTCAN), Santander, Spain.
Advances in Experimental Medicine and Biology
|July 4, 2026
Summary
Phosphorylation analysis is challenging but new enrichment materials improve phosphoproteomics. Advanced nanoparticles and MXenes enhance selectivity and efficiency for mass spectrometry platforms.
Area of Science:
- Biochemistry
- Proteomics
- Cellular Biology
Background:
- Phosphorylation is a key post-translational modification regulating cellular processes.
- Analyzing phosphoproteins is difficult due to low abundance and complex biological samples.
- Mass spectrometry-based phosphoproteomics requires effective enrichment strategies.
Purpose of the Study:
- To review recent advancements in phosphoprotein enrichment materials.
- To highlight innovations for enhanced phosphoproteomics analysis.
- To discuss the application of novel materials in quantitative proteomics.
Main Methods:
- Review of existing and emerging phosphoprotein enrichment strategies.
- Discussion of affinity chromatography techniques (IMAC, MOAC).
- Exploration of novel nanomaterials (metal nanoparticles, MXenes, carbon nanostructures) for enrichment.
Main Results:
- Advanced materials offer high surface areas, tunable chemistry, and stability for improved enrichment.
- Nanomaterials demonstrate enhanced efficiency and selectivity in phosphopeptide isolation.
- Innovations in enrichment materials significantly increase the depth and precision of phosphoproteomics.
Conclusions:
- Novel phosphoprotein enrichment materials are crucial for advancing quantitative proteomics.
- Nanomaterials show great promise for overcoming analytical challenges in phosphoproteomics.
- Continued development of enrichment strategies will improve understanding of phosphorylation in signaling and disease.
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