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Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...

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Updated: Jul 7, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Solid-Phase Glycolipid Synthesis Expedites Liposome Functionalization.

Marilet Sigler^1,2, Calvin Hon³, Kai G Pohl³

¹Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Am Muehlenberg 1, 14476 Potsdam, Germany.

Journal of the American Chemical Society

|July 6, 2026

Summary

This summary is machine-generated.

This study introduces a solid-phase synthesis platform for creating diverse glycolipids, crucial for vaccine delivery. The new method enables efficient immune cell targeting via functionalized nanoparticles.

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Last Updated: Jul 7, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

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Published on: January 11, 2017

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

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High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

Area of Science:

Carbohydrate Chemistry
Immunology
Materials Science

Background:

Glycolipids are key in immune modulation and vaccine development.
Current synthetic methods for glycolipids are complex and inefficient, limiting their accessibility.

Purpose of the Study:

To develop a unified solid-phase platform for rapid glycolipid synthesis.
To enable systematic diversification of glycolipid structures for immune applications.

Main Methods:

Automated glycan assembly and iterative amide couplings on a solid support.
On-resin generation of mannan- and Gb3-derived glycolipids.
Construction of glyco-functionalized liposomal nanoparticles.

Main Results:

A modular solid-phase platform for diverse glycolipid synthesis was established.
Glycolipid-functionalized liposomes demonstrated efficient uptake by antigen-presenting cells.
Biological performance depended on both glycan structure and lipid anchor design.

Conclusions:

The developed platform facilitates rapid and versatile synthesis of complex glycolipids.
Solid-phase synthesized glycolipids are effective for targeting immune cells via liposomal nanoparticles.
Glycolipid structure-function relationships in immune targeting were elucidated.