[Reconstruction of tetrodotoxin-dependent passive transport structures in liposomes]

Proteoliposomes were obtained from cholate solubilized components of membrane fraction and crude phospholipid fraction from the calf brain. Permeability of the proteoliposomes was studied using Na22 and Rb86 tracers. Veratrine was shown to activate and tetrodotoxin to inhibit Na22 efflux from proteoliposomes, while Rb86 efflux was not affected by the drugs used. These results demonstrate reconstitution of tetrodotoxin-dependent structure inducing selective sodium passive transport in artificial membranes.