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Updated: Jul 7, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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An Integrated Micromirror System for High-Throughput Multi-Parametric Cell Imaging.

Yingchao Meng1, Xiaobao Chao1,2, Andrew J deMello1

  • 1Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, Zürich, Switzerland.

Small Methods
|July 6, 2026
PubMed
Summary
This summary is machine-generated.

A new sheathless optofluidic imaging flow cytometry (optoIFC) platform simplifies device production using microfabricated mirrors. This high-throughput system enables precise cell analysis and mapping of intracellular heterogeneity in large cell populations.

Keywords:
fabricationfluorescencemicrofluidicsnanoscopic scalephotolithographystereolithographystroboscopetaxonomy

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Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Area of Science:

  • Biophotonics
  • Microfluidics
  • Cellular Imaging

Background:

  • Conventional imaging flow cytometry offers high-content analysis but relies on complex optics.
  • Optofluidic integration simplifies and miniaturizes imaging flow cytometers while improving performance.

Purpose of the Study:

  • To present a novel sheathless optofluidic imaging flow cytometry (optoIFC) platform.
  • To demonstrate simplified fabrication and enhanced imaging capabilities for high-throughput cell analysis.

Main Methods:

  • Developed a sheathless optoIFC platform integrating microfluidics and microfabricated mirrors.
  • Utilized two-photon stereolithography for single-step mirror fabrication within microfluidic channels.
  • Employed stroboscopic fluorescence illumination for high-throughput imaging.

Main Results:

  • Achieved simultaneous two-color fluorescence and brightfield imaging.
  • Demonstrated imaging throughputs exceeding 3,000 cells per second.
  • Successfully investigated TNF-α-induced NF-κB nuclear translocation in HL-60 cells.

Conclusions:

  • The optoIFC platform simplifies device production and enhances imaging performance.
  • Enables precise cell sizing and high-content mapping of intracellular heterogeneity.
  • Offers a powerful tool for high-throughput analysis of large, heterogeneous cell populations.