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Engineering glyphosate-resistant watermelon by prime editing
Shouwei Tian1, Yu Lu2, Jingchao Chen3
1State Key Laboratory of Vegetable Biobreeding, National Engineering Research Center for Vegetables, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China.
None:
Editing the watermelon EPSPS gene using a prime editing platform with visible markers created a non-transgenic glyphosate-resistant line. These robust heterozygous mutants tolerate field-level herbicide doses without growth penalties, serving as ideal parental lines for crop breeding.
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