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Updated: Jul 7, 2026

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

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Published on: September 7, 2015

Engineering glyphosate-resistant watermelon by prime editing.

Shouwei Tian¹, Yu Lu², Jingchao Chen³

¹State Key Laboratory of Vegetable Biobreeding, National Engineering Research Center for Vegetables, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China.

Journal of Integrative Plant Biology

|July 6, 2026

Summary

This summary is machine-generated.

Prime editing created non-transgenic glyphosate-resistant watermelon. These robust mutants tolerate herbicides, making them ideal for crop breeding programs.

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Area of Science:

Plant genetics
Molecular biology
Agricultural science

Background:

Glyphosate resistance is crucial for weed management in agriculture.
Traditional breeding methods for herbicide resistance can be time-consuming.
Gene editing offers precise and efficient ways to introduce desirable traits.

Purpose of the Study:

To develop a non-transgenic glyphosate-resistant watermelon line using prime editing.
To assess the herbicide tolerance and growth characteristics of the edited lines.
To evaluate the potential of these lines as parental stock for crop breeding.

Main Methods:

Utilized a prime editing platform to target the EPSPS gene in watermelon.
Incorporated visible markers to facilitate the identification of edited cells.
Grew edited and control lines under field-level herbicide application.
Evaluated plant growth and survival rates.

Main Results:

Successfully generated heterozygous watermelon mutants with edits in the EPSPS gene.
These non-transgenic mutants exhibited significant glyphosate resistance.
Edited lines tolerated field-level herbicide doses without observable growth penalties.
Mutants showed no negative impact on growth, indicating robustness.

Conclusions:

Prime editing is an effective tool for creating non-transgenic herbicide-resistant crops.
The developed glyphosate-resistant watermelon lines are suitable for breeding applications.
This approach offers a rapid and precise method for enhancing crop resilience to herbicides.