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Expanding the C. elegans toolkit with gonad explants.

Réda M Zellag^1,2, Eric Cheng¹, Abigail R Gerhold¹

¹Department of Biology, McGill University, 1205 avenue Docteur Penfield, Montréal, QC, H2A 1B1, Canada.

Development (Cambridge, England)

|July 6, 2026

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Extruded C. elegans gonads serve as a viable tissue explant for studying germline development. This system allows live-cell imaging and drug treatment to investigate dynamic events in animal development.

Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

Animal development involves complex, coordinated pathways.
Tissue explants offer simplified models for studying dynamic biological events.
Investigating C. elegans germline development requires robust experimental systems.

Purpose of the Study:

To establish and validate extruded C. elegans gonads as a functional tissue explant system.
To demonstrate the utility of this system for live-cell imaging and drug-based mechanistic studies.
To decipher mechanisms governing C. elegans germline development.

Main Methods:

Extrusion of C. elegans gonads to create tissue explants.
Live-cell imaging to observe cellular processes within explants.

Keywords:

Caenorhabditis elegans ex vivo culture Drug treatment Gonad development Tissue explant

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Treatment of explants with nocodazole, a microtubule-depolymerizing drug.

Main Results:

C. elegans gonad explants maintain key in vivo developmental processes like mitosis, meiosis, apoptosis, and gametogenesis.
Explants exhibit acute sensitivity to nocodazole treatment, indicating preserved cellular responses.
The system successfully supports combined live-cell imaging and drug perturbation.

Conclusions:

Extruded C. elegans gonads represent a tractable and valuable model for studying germline development.
This explant system facilitates mechanistic investigations into dynamic cellular events.
The combination of live imaging and drug treatment in this model advances understanding of developmental processes.