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Updated: Jul 7, 2026
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Inducement and Evaluation of a Murine Model of Experimental Myopia
Published on: January 22, 2019
Dual-Hit Myopia Mechanism Unveiled by Multi-Omics: Opn1mw Deficiency Primed the Retina for Exaggerated Response to
Lin Ye1,2, Xianglian Li1, Xiuyu Mao1
1Department of Ophthalmology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
Investigative Ophthalmology & Visual Science
|July 6, 2026
Summary
Cone opsin (OPN1MW) dysfunction leads to reduced dopamine and increased myopia risk. Targeting dopamine D1 receptors may protect against environmentally induced myopia.
Area of Science:
- Ophthalmology and Vision Science
- Genetics and Molecular Biology
- Neuroscience
Background:
- Cone opsins, particularly OPN1MW, are crucial for visual development.
- Genetic variants in OPN1MW are linked to high myopia in humans.
- Understanding the molecular mechanisms linking opsin dysfunction to myopia is essential.
Purpose of the Study:
- To investigate how M-opsin dysfunction influences molecular pathways and functional susceptibility to myopia.
- To explore the role of dopaminergic signaling in myopia development associated with M-opsin deficiency.
Main Methods:
- Transcriptome, metabolome, and proteome sequencing in M-opsin knockout (MKO), S-opsin knockout (SKO), and wild-type (WT) mice.
- Induction of lens-induced myopia (LIM) in MKO and WT mice.
- Pharmacological intervention using a dopamine D1 receptor agonist (SKF38393) in MKO mice.
Main Results:
- MKO and SKO mice exhibited hyperopic shifts; MKO mice showed exacerbated myopia under LIM conditions with reduced retinal dopamine.
- Proteomic analysis revealed shared dopaminergic synapse alterations in M-opsin deficiency and LIM.
- A network involving TFAM, KDM5C, and SMN1 was downregulated in MKO mice and further affected by LIM; SKF38393 treatment rescued myopia and normalized TFAM/SMN1 levels.
Conclusions:
- M-opsin dysfunction contributes to myopia susceptibility via reduced retinal dopaminergic tone, supporting a gene-environment interaction model.
- A dysregulated TFAM/KDM5C/SMN1 network may indicate retinal vulnerability to environmental myopia triggers.
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