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Related Experiment Video

Updated: Jul 7, 2026

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
09:59

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Published on: January 29, 2019

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Jacob Doon-Ralls1,2, Sophia Mayone1, Xilal Y Rima1,3

  • 1Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 W. Woodruff Ave., Columbus, 43210, Ohio, USA. reategui.8@osu.edu.

Lab on a Chip
|July 6, 2026
PubMed
Summary

Researchers developed a new platform for precisely studying neutrophil extracellular trap (NET) formation at the single-cell level. This tool helps understand NETosis, crucial for immunity and inflammation, and aids in developing new therapies.

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Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils
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Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils

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Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
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Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

Related Experiment Videos

Last Updated: Jul 7, 2026

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
09:59

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Published on: January 29, 2019

Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils
07:19

Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils

Published on: December 1, 2023

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Neutrophils are key innate immune cells crucial for pathogen defense.
  • Neutrophil extracellular trap (NET) formation is vital in inflammation but poorly understood.
  • Existing tools lack the resolution to study diverse NETosis forms (vital and suicidal).

Purpose of the Study:

  • To introduce a novel, tunable micropatterned platform for high-resolution, single-cell analysis of NETosis.
  • To enable spatial control over neutrophil behavior and NETosis outcomes.
  • To facilitate investigation into immune heterogeneity and targeted therapy development.

Main Methods:

  • Utilized photopatterned bacterial extracellular vesicles (bEVs) on functionalized surfaces.
  • Engineered micropatterns to control human peripheral blood neutrophil alignment.
  • Applied phorbol 12-myristate 13-acetate (PMA) for uniform suicidal NETosis induction.
  • Enabled live-cell imaging for intracellular investigations.

Main Results:

  • Demonstrated controlled neutrophil alignment and spatial precision in NETosis studies.
  • Showcased engineered patterns influencing key NETosis outcomes.
  • Confirmed PMA stimulation induces uniform suicidal NETosis.
  • Validated the platform's flexibility for various patterning and stimulation conditions.

Conclusions:

  • The novel platform allows precise quantification and manipulation of NETosis at the single-cell level.
  • This system opens new avenues for studying immune cell heterogeneity.
  • Facilitates the development of targeted immunotherapies by understanding NETosis pathways.