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Updated: Jul 7, 2026
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Studying the Effects of Inhaled Environmental Pollutants on Olfactory Function in Mice
Published on: September 13, 2024
Eosinophil cationic protein-driven type 2 inflammation impairs olfactory function in a murine model.
Zhong Zheng1, Liufeiyan Fan1, Haofei Bai1
1Department of Otolaryngology, Xijing Hospital, The Air Force Military Medical University, Xi'an, Shaanxi, China.
Summary
This study developed a mouse model for eosinophil-induced olfactory dysfunction (EIOD) using eosinophil cationic protein (ECP). The model demonstrated reduced olfactory function and inflammation, offering insights into ECRS.
Area of Science:
- Immunology
- Otorhinolaryngology
- Neuroscience
Background:
- Eosinophilic chronic rhinosinusitis (ECRS) is linked to olfactory dysfunction (OD).
- Mechanisms of OD in ECRS are not fully understood.
- A rapid and effective murine model is needed to study EOD.
Purpose of the Study:
- To develop a murine model of eosinophil-induced olfactory dysfunction (EIOD) using intranasal eosinophil cationic protein (ECP).
- To assess the model's efficacy through behavioral, morphological, and molecular analyses.
Main Methods:
- Male mice received intranasal ECP for 10 or 20 days.
- Olfactory behavior, histology, cytokine analysis, and gene expression profiling were performed.
- RNA sequencing was used to analyze inflammatory pathways.
Main Results:
- The ECP-treated mice showed significant olfactory dysfunction and reduced olfactory epithelium thickness.
- A decrease in olfactory sensory neurons and increased type 2 inflammatory cytokines were observed.
- Olfactory function and mucosa showed recovery upon ECP cessation, with identified inflammatory pathway alterations.
Conclusions:
- An effective murine model for EIOD was successfully established and validated.
- This model provides a valuable tool for studying ECRS-associated OD pathogenesis.
- The model can aid in developing therapeutic interventions for ECRS-related olfactory loss.
