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Related Concept Videos

Necrosis01:16

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Maturation of Endosomes01:28

Maturation of Endosomes

The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Inflammation01:38

Inflammation

Overview
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...

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Related Experiment Video

Updated: Jul 7, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Senescent cells accumulate lipid droplets.

Noa Rachmian-Cooper1, Riva Shmulevich1, Hagay Akiva1

  • 1Department of Molecular Cell Biology, Weizmann Institute of Science, 7610001, Rehovot, Israel.

Aging
|July 6, 2026
PubMed
Summary
This summary is machine-generated.

Senescent cells accumulate lipid droplets, contributing to Alzheimer's disease pathology. This study identifies senescent microglia with lipid droplets as a key cell population driving disease progression.

Keywords:
Alzheimer’s diseaseaginglipid dropletsmetabolismsenescence

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Related Experiment Videos

Last Updated: Jul 7, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Area of Science:

  • Cellular senescence
  • Metabolic reprogramming
  • Neurodegenerative diseases

Background:

  • Senescent cells (SnCs) are metabolically active and exhibit a Senescence-Associated Secretory Phenotype (SASP).
  • Key metabolic alterations in SnCs include increased glycolysis, altered mitochondrial function, and dysregulated lipid metabolism.
  • The precise contribution of these metabolic changes to SnC pathophysiology remains unclear.

Purpose of the Study:

  • To investigate the role of metabolic changes, particularly lipid metabolism, in senescent cells.
  • To determine if lipid droplet accumulation in senescent cells contributes to Alzheimer's disease (AD) pathology.
  • To identify potential shared characteristics between lipid droplet-containing microglia and senescent microglia in AD.

Main Methods:

  • Metabolic profiling of senescent cells.
  • Analysis of lipid droplet markers in senescent primary human fibroblasts.
  • Investigation of senescent microglia and lipid droplet markers in a mouse model of AD.
  • Single-nucleus analysis of brain cells from AD patients.

Main Results:

  • Senescent cells show elevated glycolytic metabolites and increased lipid metabolites, specifically triacylglycerol derivatives.
  • Senescent cells accumulate lipid droplets (LDs).
  • Senescent microglia in an AD mouse model and senescent brain cells in AD patients exhibit upregulated LD markers, indicating a pathological role.

Conclusions:

  • Lipid droplet accumulation is a key metabolic feature of senescent cells.
  • Senescent microglia characterized by lipid droplet accumulation are implicated in Alzheimer's disease pathology.
  • These findings suggest that lipid droplet-laden senescent microglia represent a critical cell population driving AD progression.