Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunodominant B-cell epitopes for precision surveillance of <i>Plasmodium vivax</i> malaria.

Emerging microbes & infections·2026
Same author

The association of oral health with anxiety symptoms among older adults in China: a cross-sectional study.

BMC geriatrics·2026
Same author

Mycoplasma pneumoniae infection impairs asthma control in pediatric patients and exacerbates allergic airway inflammation.

Respiratory research·2026
Same author

Longitudinal associations between exercise duration and sleep quality and poor sleep outcomes among college students: a latent growth curve modeling study.

BMC psychology·2026
Same author

Associations of chronic pain and genetic risks with incident atrial fibrillation: a prospective cohort study.

BMC cardiovascular disorders·2026
Same author

Self-assembled chamber-like cardiac organoids for modeling cardiac chamber formation and cardiotoxicity assessment.

Nature communications·2026
Same journal

Switching Site Selectivity in Alkoxyamine Hydration: From Lone-Pair Direction to Solvent Network Dominance.

Journal of the American Chemical Society·2026
Same journal

A Topotactic Leap: 2D Layers to 3D Large-Pore Zeolite.

Journal of the American Chemical Society·2026
Same journal

Enhanced Hydrogen Evolution over Single-Atom Catalysts via Electrostatic Polarization in Contact-electro-catalysis.

Journal of the American Chemical Society·2026
Same journal

Tumor Acidity-Activatable Ionizable Lipid Nanoparticles for Selective Oncolytic Therapy.

Journal of the American Chemical Society·2026
Same journal

Alternating Magnetic Field Promotes Ammonia Cracking by Disrupting the Sabatier Limitation of Ruthenium Catalytic Species.

Journal of the American Chemical Society·2026
Same journal

Bulk Ferromagnetic Icosahedral Quasicrystals without Rapid Quenching.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jul 8, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Engineering Organelle-Gated Reporters for Imaging Subcellular Enzyme Activity in Living Cells.

Yang Shen1, Wei Li1,2, Hanqing Zhao3

  • 1State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

Journal of the American Chemical Society
|July 6, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed new imaging tools to visualize enzyme activity within specific cell parts. This revealed distinct roles for mitochondrial and ER enzymes in cancer, offering new therapeutic strategies.

More Related Videos

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Related Experiment Videos

Last Updated: Jul 8, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • Enzymes are crucial for cellular functions, but their roles at specific subcellular locations are poorly understood due to a lack of precise imaging tools.
  • Understanding enzyme activity within organelles like mitochondria and the endoplasmic reticulum is key to deciphering cellular processes.

Purpose of the Study:

  • To develop novel fluorogenic reporters for spatiotemporally resolved imaging of enzyme activity in specific subcellular compartments.
  • To investigate the physiological roles of enzymes in mitochondria and endoplasmic reticulum using the developed reporters.

Main Methods:

  • Designed fluorogenic reporters with quenched fluorescence, enzyme-specific substrates, and bioorthogonal cage groups.
  • Utilized sequential uncaging and enzymatic activation for signal restoration within targeted organelles (mitochondria, ER).
  • Applied reporters to visualize leucyl aminopeptidases (LAP), γ-glutamyl transferases (GGT), and monoamine oxidase A (MAO-A) in live cells.

Main Results:

  • Visualized compartment-dependent LAP activity, with mitochondrial LAP maintaining redox homeostasis and ER LAP promoting immune-mediated tumor cell killing.
  • Demonstrated that cisplatin and camptothecin amplified both mitochondrial and ER LAP activities, with opposing effects on tumor progression.
  • Identified distinct subcellular enzyme functions influencing cell survival and immune response.

Conclusions:

  • The developed reporters enable precise imaging of enzyme activity within organelles, revealing novel biological functions.
  • Compartment-specific enzyme activities play critical roles in cellular processes, including cancer progression and immune response.
  • This platform provides a versatile framework for dissecting compartmentalized enzymology and developing targeted therapies.