Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

Overview
Translation in Prokaryotes01:29

Translation in Prokaryotes

Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Origin of Life in the Light of Evolution.

ArXiv·2026
Same author

Rapid evolution of lncRNAs introduces novel regulatory inputs into ancestral cancer pathways.

Science advances·2026
Same author

Virological investigation and comparative genomic analysis of elephant endotheliotropic herpesvirus 1B infection in an Australian captive herd of Asian elephants (Elephas maximus).

PloS one·2026
Same author

Cross-domain transfer of trehalose biosynthesis genes contributes to adaptation in high-altitude environments.

National science review·2026
Same author

Emergence of genetic sex determination in an environmentally sex-determined animal.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Biocontrol potential and molecular basis of predation in a marine raptorial ciliate.

The ISME journal·2026
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Jul 8, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Translation accuracy in E. coli.

Ryan Stikeleather1, Farhan Ali1, Wei-Chin Ho1,2

  • 1Biodesign Center for Mechanisms of Evolution, Arizona State University, S McAllister Ave., Tempe, AZ 85281, United States.

Nucleic Acids Research
|July 6, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a proteome-wide mass spectrometry method to measure translation errors, finding overall error rates are low and not significantly different between ribosomal variants. Unique mistranslation profiles, not overall accuracy, distinguish variants, challenging previous assumptions.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
06:56

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Published on: March 24, 2023

Related Experiment Videos

Last Updated: Jul 8, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
06:56

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Published on: March 24, 2023

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Accurate protein synthesis (translation) is vital for life.
  • Previous methods for measuring translation errors were limited, often focusing on single codons.
  • A comprehensive understanding of translation fidelity across the entire proteome is lacking.

Purpose of the Study:

  • To develop and apply a proteome-wide method for directly estimating amino acid substitution rates.
  • To characterize mistranslation rates and profiles per amino acid and codon.
  • To re-evaluate translation fidelity in ribosomal variants and test the translational-accuracy hypothesis of codon usage.

Main Methods:

  • Utilized mass spectrometry data for a proteome-wide analysis.
  • Developed a novel approach to estimate pairwise amino acid substitution rates.
  • Applied the method to analyze ribosomal variants of Escherichia coli.

Main Results:

  • Overall translation error rates were estimated at 2 per 1000 amino acids, with no significant differences found between ribosomal variants.
  • Each ribosomal variant displayed distinct mistranslation profiles, challenging the notion of a universally error-prone variant.
  • Codons favored in highly expressed genes were not found to be translated with higher accuracy, contradicting the translational-accuracy hypothesis.

Conclusions:

  • Proteome-wide measures are essential for accurately characterizing translation fidelity.
  • Single-codon reporter assays may overestimate or misrepresent overall translation error rates.
  • The translational-accuracy hypothesis of codon usage is not supported by these findings.