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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jul 8, 2026

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

SMART (Shuimu Automated Reconstruction Technology): An Integrated Computational Platform for Streamlining

Haibin Liu1, Yangyu Wu2, Wenpeng Zhang3

  • 1Shuimu BioSciences Ltd.; liuhaibin@shuimubio.com.

Journal of Visualized Experiments : Jove
|July 6, 2026
PubMed
Summary

We developed SMART, an integrated cryo-electron microscopy (cryo-EM) software platform, streamlining data collection, 3D reconstruction, and atomic model building for structural biology. This automated workflow enhances efficiency and accessibility for determining complex protein structures.

More Related Videos

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Related Experiment Videos

Last Updated: Jul 8, 2026

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Current cryo-electron microscopy (cryo-EM) workflows are fragmented, requiring multiple software packages and manual intervention.
  • This complexity hinders efficient structure determination and limits accessibility for researchers.

Purpose of the Study:

  • To introduce SMART (Shuimu Automated Reconstruction Technology), an integrated software platform for cryo-EM.
  • To provide a unified, browser-accessible interface combining automated data collection, image processing, 3D reconstruction, and atomic model building.

Main Methods:

  • SMART integrates DataSmart (automated data collection), CryoSmart (image processing and 3D reconstruction), and ModelSmart (deep-learning-based model building).
  • The protocol details automated specimen navigation, data acquisition, motion correction, CTF estimation, particle picking, 2D classification, 3D refinement, map enhancement, and atomic model generation.
  • The workflow was applied to determine the structure of human TRPML1, a Ca2+ channel.

Main Results:

  • Achieved a global resolution of 2.38 Å for human TRPML1 using the complete SMART workflow.
  • Demonstrated successful integration and sequential application of DataSmart, CryoSmart, and ModelSmart modules on a single dataset.
  • Validated the platform's capability for high-resolution structure determination.

Conclusions:

  • SMART offers a streamlined, automated, and integrated solution for cryo-EM data processing and structure determination.
  • The platform enhances efficiency and accessibility, catering to users with diverse cryo-EM experience levels.
  • Facilitates rapid and robust atomic model building for biological macromolecules.