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Updated: Jul 8, 2026

Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation

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A Three-Dimensional Culture Model Supporting Human Secondary-to-Antral Follicle Development In Vitro.

Jianfei Gong¹, Zhiyun Zhu², Jinze Yang¹

¹State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital; National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education; Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality.

Journal of Visualized Experiments : Jove

|July 6, 2026

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Researchers developed a novel 3D culture model for human ovarian follicle development. This system successfully mimics in vivo growth, supporting oocyte maturation and revealing key receptor expression patterns for future research.

Area of Science:

Reproductive Biology
Developmental Biology
Cell Biology

Background:

Human ovarian folliculogenesis is complex and difficult to study in vivo.
Existing in vitro models fail to replicate the dynamic spatiotemporal development of ovarian follicles.

Purpose of the Study:

To develop and validate a 3D culture model for human follicle development from secondary to antral stages.
To assess the model's ability to recapitulate in vivo morphological and cellular events.
To provide a platform for studying human folliculogenesis and reproductive toxicology.

Main Methods:

Development of a novel 3D culture system for human ovarian follicles.
Monitoring of morphological changes and follicular growth over 20 days.
Immunofluorescence analysis of gonadotropin receptor expression (FSHR, LHR) in somatic cells.

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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

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Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation

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Published on: May 9, 2025

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Published on: March 17, 2015

Main Results:

The 3D model supported sustained follicular growth, diameter expansion, and antral cavity formation.
Viable oocytes at the germinal vesicle (GV) stage were successfully retrieved.
Distinct FSHR and LHR expression patterns were observed in granulosa-like and theca-like cells, respectively.

Conclusions:

The 3D culture model effectively recapitulates key stages of human folliculogenesis in vitro.
This model serves as a valuable tool for mechanistic studies and reproductive toxicology.
The findings offer a reference for optimizing in vitro follicle culture systems.