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Transcriptomics-guided mco overexpression enhances biogenic amine reduction by Lactobacillus sakei MDJ6 in vitro and
Jiasheng Lu1, Huiping Wang1, Mingzhu Zhang1
1Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.
Abstract:
This study aimed to elucidate the transcriptional response of Lactobacillus sakei MDJ6 to putrescine stress and to enhance its biogenic amine (BA)-degrading capacity via mco overexpression. Transcriptomic analysis identified 105 differentially expressed genes (DEGs) primarily associated with membrane transport and metabolic remodeling. In addition, the engineered strain L. sakei MDJ6-mco was constructed, exhibiting intracellular and extracellular apparent multicopper oxidase activities in crude enzyme extracts of 171.18 and 349.01 U/g in vitro, representing significant increases of 2.05-fold and 3.16-fold, respectively, compared to L. sakei MDJ6 (P < 0.05). In a dry sausage model, inoculation with L. sakei MDJ6-mco resulted in the lowest total BA content on day 9 (64.31 mg/kg), corresponding to an overall BA reduction rate of 76.81%, compared with 55.87% in the LS treatment (P < 0.05). By integrating transcriptomic insights with targeted genetic engineering, this study investigated the putative response pattern of L. sakei MDJ6 to putrescine stress and showed that overexpression of the mco gene enhanced BA-degrading capacity of L. sakei MDJ6.
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