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Related Experiment Video

Updated: Jul 8, 2026

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

Common carp USP30s restrict SVCV replication by attenuating mitophagy.

Chen Li¹, Songjie Qi¹, Mengxi Zhang¹

¹Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang, 453007, China.

Fish & Shellfish Immunology

|July 6, 2026

Summary

Common carp USP30 proteins (CcUSP30s) counteract excessive mitophagy during viral infection. This study reveals CcUSP30s preserve mitochondrial integrity, limiting viral replication and offering potential aquaculture strategies.

Keywords:

Antiviral Cyprinus carpio Mitophagy SVCV USP30

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Published on: May 19, 2023

Area of Science:

Immunology
Cell Biology
Virology

Background:

Mitophagy, a selective autophagic process, is crucial for antiviral immunity by clearing damaged mitochondria.
The deubiquitinating enzyme USP30 regulates mitophagy in mammals, but its role in teleost antiviral defense is unknown.

Purpose of the Study:

To investigate the function of USP30 homologs (CcUSP30-A and CcUSP30-B) in common carp (Cyprinus carpio) antiviral immunity.
To determine the role of CcUSP30s in regulating mitophagy during spring viremia of carp virus (SVCV) infection.

Main Methods:

Identification and characterization of CcUSP30-A and CcUSP30-B in common carp.
In vitro analysis of SVCV infection, mitophagy induction (PINK1-Parkin pathway), and CcUSP30s' effects on viral replication.
Overexpression studies of CcUSP30-A and CcUSP30-B to assess their impact on mitochondrial proteins, mitophagy, and SVCV replication.

Main Results:

SVCV infection induced PINK1-Parkin-dependent mitophagy, which the virus exploited for replication.
Overexpression of CcUSP30-A and CcUSP30-B increased mitochondrial protein levels, reduced SVCV-induced mitophagy, and inhibited viral replication.
CcUSP30-B exhibited stronger anti-mitophagy and anti-viral activities compared to CcUSP30-A.

Conclusions:

CcUSP30s antagonize the PINK1-Parkin pathway, mitigating excessive mitophagy and limiting SVCV replication.
USP30 acts as an antiviral regulator in teleosts by maintaining mitochondrial integrity.
CcUSP30s represent potential therapeutic targets for controlling SVCV in aquaculture.