Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sub-millimeter in-fiber Michelson interferometer with intrinsic Vernier amplification enabled by a non-adiabatic dual-tapered microsphere structure.

Optics express·2026
Same author

Electrically Tunable Meta-Waveplate Enabled by Sb<sub>2</sub>Se<sub>3</sub>-Heterogeneously Integrated Piezoelectric MEMS Mirror.

Micromachines·2026
Same author

A Spectral Analysis Method Based on Direct Absorption Intensity Reconstruction for Rapid Real-Time CO Detection.

Analytical chemistry·2026
Same author

Flexible microwave pulses regulation in self-mode-locking optoelectronic oscillator.

Optics express·2025
Same author

Transient hydraulic pressure sensor based on single-hole-dual-core fiber Bragg grating.

Optics express·2025
Same author

Laser spectroscopy applied in radiocarbon dating with the bomb peak.

Optics express·2025

Related Experiment Video

Updated: Jul 8, 2026

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

TDLAS-based antimicrobial susceptibility testing using microbial CO2 evolution and validation in Gram-positive and

Lingjie Kong1, Wen Liu1, Yiwen Shang2

  • 1Zhejiang Normal University, The Key Laboratory of Optical Information Detection and Display Technology of Zhejiang, Jinhua 321004, China.

Journal of Applied Microbiology
|July 6, 2026
PubMed
Summary

A novel non-invasive antimicrobial susceptibility testing (AST) method using Tunable Diode Laser Absorption Spectroscopy (TDLAS) accurately determines minimum inhibitory concentrations (MICs) and provides detailed bacterial growth kinetics. This low-cost approach shows potential for clinical settings and resource-limited environments.

Keywords:
AMRantibioticsantimicrobial resistanceantimicrobialsbacteria

More Related Videos

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

Related Experiment Videos

Last Updated: Jul 8, 2026

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Antimicrobial susceptibility testing (AST) is crucial for effective treatment and combating antimicrobial resistance (AMR).
  • Traditional AST methods are time-consuming and lack real-time microbial growth data.
  • A need exists for rapid, non-invasive, and cost-effective AST solutions.

Purpose of the Study:

  • To develop and validate a universal, non-invasive AST method utilizing Tunable Diode Laser Absorption Spectroscopy (TDLAS).
  • To quantitatively determine minimum inhibitory concentrations (MICs) by monitoring microbial CO2 evolution.
  • To assess the method's stability, accuracy, and applicability to diverse bacterial strains.

Main Methods:

  • Monitoring CO2 production in sealed culture bottles via TDLAS.
  • Establishing a calibration curve correlating threshold time (TT) with total viable count (TVC).
  • Developing a dose-response model for MIC determination and validating against CLSI standards.

Main Results:

  • The TDLAS method yielded ampicillin MIC values for *Staphylococcus sciuri* concordant with standard broth microdilution.
  • Excellent method stability was demonstrated with a coefficient of variation (CV) of 2.75% over four replicates.
  • Successful application to clinical isolates (*Staphylococcus aureus*, *Escherichia coli*, *Enterobacter cloacae*) confirmed broad applicability.

Conclusions:

  • The TDLAS-based AST is a universal, non-invasive, and low-cost method yielding accurate MICs.
  • It offers superior insights into bacterial growth dynamics under antibiotic stress compared to traditional methods.
  • The technology holds significant promise for clinical diagnostics and resource-limited settings.