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Related Concept Videos

Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Antifungal Agents01:15

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry01:20

Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry

Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Lipids as Anchors01:32

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...

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Related Experiment Video

Updated: Jul 8, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Strain-dependent bioactive-lipid interactions govern oxidative stability and antimicrobial functionality in Lentinula

Sujata Makkar1, Kiran Nehra1, Sudheer Kumar Annepu2

  • 1Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Haryana 131039, India.

Food Research International (Ottawa, Ont.)
|July 6, 2026
PubMed
Summary

Edible mushroom strains vary in their ability to preserve food by preventing spoilage. Strain selection is key for identifying Lentinula edodes with optimal antioxidant and antimicrobial properties for natural food preservation.

Keywords:
Lentinula edodesantimicrobial activityantioxidant capacitybioactive metabolitesfood preservationoxidative stabilitystrain variability

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Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
12:57

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Published on: September 16, 2013

Related Experiment Videos

Last Updated: Jul 8, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
12:57

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Published on: September 16, 2013

Area of Science:

  • Food Science
  • Mycology
  • Biochemistry

Background:

  • Edible mushrooms are explored for natural food preservation due to antioxidant and antimicrobial properties.
  • Evaluating these properties requires an integrated, strain-resolved biochemical framework.

Purpose of the Study:

  • To compare five Indian Lentinula edodes strains for bioactive profiles influencing lipid stability, antioxidant buffering, and antimicrobial efficacy.
  • To understand how strain-dependent traits impact food preservation potential.

Main Methods:

  • Fatty acid profiling and lipid peroxidation assays (malondialdehyde levels).
  • Antioxidant capacity assessment (radical-scavenging, metal-chelating, ascorbic acid, total antioxidant capacity).
  • Antimicrobial activity testing (MIC, MBC/MFC) against bacteria and Candida albicans.

Main Results:

  • Significant strain-dependent variations in fatty acid profiles, lipid peroxidation, and antioxidant capacity were observed.
  • All strains showed antimicrobial activity, with notable strain-specific differences.
  • Strain DMRO-356 exhibited high PUFA content, low lipid peroxidation, strong antioxidant capacity, and enhanced antimicrobial activity.

Conclusions:

  • Functional food preservation properties in Lentinula edodes result from coordinated interactions between lipid composition and bioactive metabolites.
  • Strain selection is a critical factor for optimizing the use of L. edodes in natural food preservation systems.