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Association of MTHFR and platelet-related blood cell ratio parameters with ischemic stroke in Gansu population
Youli Zhao1, Xiaojuan Li2, Yijie Wang3
1Department of Clinical Medical Laboratory, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730030, China.
Objective:
To investigate the association of MTHFR polymorphisms with the susceptibility to ischemic stroke (IS) in Gansu population in China, and the predictive value of platelet-related ratios for the risk of IS in different genotypes.
Methods:
We enrolled 121 IS patients admitted between June and December 2022 and 121 recruited from the same source population healthy controls. Clinical data were collected, and peripheral blood was analyzed for routine parameters. Genotyping for MTHFR C677T and A1298C was performed using PCR fluorescence probe. Statistical analysis was conducted with SPSS Statistics 27.0.
Results:
In the Gansu population, the MTHFR C677T TT and A1298C CC genotypes were significantly associated with increased IS risk (P = 0.005, P = 0.01). Specific combined genotypes at these loci (CT/CC, TT/AC, TT/CC) elevated IS risk by 1.4-, 1.7- and 1.4-fold, respectively. In the IS group, the MPV/PLT, MPV/LY and RDW/PLT ratios were significantly elevated, while PLT/NE was lower (all P < 0.05). Additionally, patients with the MTHFR C677T TT genotype had higher NE levels than those with the CC genotype (P = 0.028), and those with the MTHFR A1298C CC genotype exhibited a higher MPV/LY ratio than AA carriers (P = 0.038). ROC analysis indicated that combining NE and MPV/LY for IS risk assessment yielded an AUC of 0.784 (95% CI: 0.726-0.842).
Conclusion:
MTHFR C677T and A1298C polymorphisms are associated with IS risk in the Gansu population. The combined assessment of NE, MPV/LY, and MTHFR C677T genotyping may offer adjunctive information for IS risk assessment, pending independent validation.
