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Related Experiment Video

Updated: Jul 8, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Specific bile acids can elicit the type-I interferon response through the cGAS-STING pathway.

Jianan He¹, Ziyan Huang¹, Caiwan Xiong¹

¹National Key Laboratory of Immune Response and Immunotherapy, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.

Cell Communication and Signaling : CCS

|July 7, 2026

Summary

Specific bile acids trigger the type-I interferon response via the cGAS-STING pathway, leading to mitochondrial DNA release. This mechanism offers potential for antiviral and antitumor therapies.

Keywords:

Bile acid Calcium flux Influenza virus Mitochondria fission STING Type-I interferon cGAS

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Last Updated: Jul 8, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Published on: September 18, 2014

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Area of Science:

Immunology
Cell Biology
Metabolomics

Background:

Bile acids are vital for lipid metabolism and immune regulation.
Their precise biological roles and mechanisms are not fully understood.

Purpose of the Study:

To investigate how specific bile acids influence cellular responses.
To elucidate the mechanistic pathways involved in bile acid-mediated immune activation.

Main Methods:

Utilized phosphoproteomics to analyze signaling pathway changes.
Investigated the role of the cGAS-STING pathway in bile acid responses.
Examined inter-organelle interactions (endolysosome, ER, mitochondria) and calcium flux.

Main Results:

Specific bile acids (DCA, CDCA, LCA) activate the type-I interferon (IFN-I) response through the cGAS-STING pathway.
Bile acids induce mitochondrial dysfunction and release of mitochondrial DNA (mtDNA).
Systemic DCA administration triggers STING-dependent IFN-I response; localized DCA shows antiviral/antitumor effects.

Conclusions:

The cGAS-STING pathway is a key mechanism for bile acid-induced IFN-I responses.
Bile acids can modulate cellular signaling, leading to mtDNA release and immune activation.
Targeting bile acid pathways offers potential therapeutic strategies for viral infections and cancer.