Lactobacillus paragasseri lg94 With High L-Lactic Acid Production and Probiotic Potential

Area of Science:

• Microbiology
• Probiotics
• Genomics

Background:

• Lactobacillus paragasseri strains are common probiotics requiring thorough characterization.
• Assessing probiotic safety and potential involves evaluating morphological, cultural, physiological-biochemical, and antagonistic properties.
• Virulence, toxigenicity, toxicity, and secondary metabolite production are key factors in probiotic safety.

Purpose of the Study:

• To determine the probiotic potential of the Lactobacillus paragasseri lg94 strain.
• To characterize its biochemical features and analyze its genomic landscape.
• To evaluate its safety for potential probiotic applications.

Main Methods:

• Isolation and identification of Lactobacillus paragasseri lg94 from human feces using MALDI Biotyper.
• Assessment of cultural, biochemical characteristics, L-lactic acid production, and resistance to bile and low pH stress.
• Genomic sequencing using Illumina NextSeq550 and analysis for antibiotic resistance, pathogenicity, and virulence determinants.

Main Results:

• Lactobacillus paragasseri lg94 demonstrated fermentation of 25 carbohydrates and high L-lactic acid production (6.41 g/L).
• The strain showed low acid stress resistance but high bile resistance.
• Genomic analysis confirmed the absence of antibiotic resistance, pathogenicity, and virulence genes, but identified genes for gassericin T, enterolysin A, and helveticin J production.

Conclusions:

• Lactobacillus paragasseri lg94 exhibits significant probiotic potential.
• Further research into its antagonistic activity and secondary metabolite production is recommended.
• The strain's safety profile supports its consideration for probiotic development.