Related Experiment Video
Updated: Jul 8, 2026
09:03
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
Published on: May 10, 2020
From Gene Function to Precision Intervention: CRISPR/Cas9 and Stem Cell-Based Strategies as Emerging
Masuma Khatun1,2, Karolina Lundin3, Timo Tuuri3
1Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 2, 00290, Helsinki, Finland. masuma.khatun@helsinki.fi.
Stem Cell Reviews and Reports
|July 7, 2026
Summary
Polyendocrine metabolic ovarian syndrome (PMOS) is a complex disorder affecting women
Area of Science:
- Reproductive Endocrinology and Metabolism
- Genetics and Genomics
- Regenerative Medicine
Background:
- Polyendocrine metabolic ovarian syndrome (PMOS) affects 18% of women globally, causing anovulatory infertility.
- Current PMOS treatments manage symptoms but not underlying molecular disruptions.
- Multi-omic studies reveal genetic links and pathway disturbances in PMOS.
Purpose of the Study:
- To explore novel therapeutic strategies for PMOS by integrating functional genomics and stem cell research.
- To investigate the potential of CRISPR/Cas9 and stem cell-based platforms for PMOS.
- To shift PMOS management towards mechanism-driven interventions.
Main Methods:
- Utilizing CRISPR/Cas9 gene editing for precise pathway interrogation.
- Employing stem cell platforms: mesenchymal stem cells (MSCs), exosomes, and gene-edited induced pluripotent stem cells (iPSCs).
- Conducting preclinical studies on MSCs and iPSC-based models for disease modeling and regeneration.
Main Results:
- MSCs and derivatives show potential in modulating inflammation, restoring ovarian structure, and improving metabolic parameters.
- iPSC models facilitate patient-specific investigation of steroidogenic and metabolic abnormalities.
- CRISPR-based functional genomics combined with stem cell research offers a promising approach for PMOS.
Conclusions:
- Integrating CRISPR-based functional genomics with stem cell research represents a paradigm shift in PMOS management.
- This approach moves beyond symptom management towards targeted, mechanism-driven interventions.
- Future research should address translational challenges for effective PMOS treatment.
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