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Related Experiment Video

Updated: Jul 9, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

Decoding bipotency: a transient regulatory state bridging totipotency and lineage commitment.

Amash Manzoor¹, Qingyi Lin², Wenjuan Li²

¹Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.

Current Opinion in Genetics & Development

|July 7, 2026

Summary

This summary is machine-generated.

Mammalian embryos resolve totipotency through a transient bipotent state, bridging embryonic and extra-embryonic lineages. This transition involves transposable elements, transcription factors, and signaling pathways, crucial for early development.

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Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

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Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Published on: May 8, 2020

Area of Science:

Developmental Biology
Stem Cell Biology
Genomics

Background:

Early mammalian embryogenesis involves a critical transition from totipotency to lineage specification.
The precise mechanisms resolving totipotency into distinct embryonic and extra-embryonic lineages are not fully understood.
A non-binary model suggests cells pass through a transient bipotent intermediate stage.

Purpose of the Study:

To synthesize recent advances in understanding the establishment, maintenance, and resolution of bipotency.
To highlight the roles of transposable elements, transcription factors, and signaling pathways in this process.
To discuss the implications of novel bipotent stem cell models for in vitro embryo development.

Main Methods:

Literature synthesis of recent research findings.
Analysis of molecular mechanisms including transposable elements, transcription factors, and signaling pathways.
Review of newly developed bipotent stem cell models.

Main Results:

Bipotency is established, maintained, and resolved through the coordinated action of transposable elements, transcription factors, and signaling pathways.
Transient bipotent intermediates are key to navigating the continuum of potency states.
Novel stem cell models offer new avenues for studying early embryonic development in vitro.

Conclusions:

Understanding bipotency resolution is crucial for deciphering early mammalian development.
The coordinated regulation of genetic and signaling factors governs the transition from totipotency.
Further research is needed to extend findings from mouse models to human embryogenesis.