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Stretching the limits: Decoding hidden biology with vibrational probes
Ziguang Yang1, Philip A Kocheril1, Adrian Colazo1
1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Abstract:
Over the past two decades, vibrational bioimaging has emerged as a powerful means of studying complex biological systems. Though early work focused on label-free imaging, the use of vibrational probes enabled highly specific imaging of metabolic incorporation and turnover, as well as providing unique opportunities for probe engineering, towards robust single-molecule vibrational imaging, super-multiplex imaging, and quantitative vibrational local sensing. Here, we review recent progress in the application of vibrational probes for biological imaging and provide forward-looking insights towards the design of novel probes for deeper functional studies of biological processes and dynamics.
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