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Updated: Jul 9, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Accelerated algal-bacterial granulation through synergistic physical-ionic stimulation: start-up efficiency and
Duanyang Shangguan1, Libin Yang1, Cheng Hou1
1College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Water Pollution Control and Green Resource Recycling, Shanghai 200092, China.
Combining a static magnetic field with magnesium ions significantly speeds up algal-bacterial granular sludge formation for wastewater treatment. This sustainable approach enhances nutrient removal and sludge stability, reducing costs and overcoming key industrial deployment challenges.
Area of Science:
- Environmental Science and Engineering
- Biotechnology
- Materials Science
Background:
- Algal-bacterial granular sludge (ABGS) offers a sustainable path for carbon-neutral wastewater treatment.
- Current industrial use of ABGS is hindered by slow granulation and unstable aggregates.
- Existing methods for enhancing ABGS struggle to balance rapid formation with structural integrity cost-effectively.
Purpose of the Study:
- To investigate the synergistic effect of a static magnetic field (SMF) and Mg2+ on accelerating ABGS formation.
- To analyze the impact of this coupled strategy on the physicochemical properties and microbial community of ABGS.
- To evaluate the techno-economic feasibility of the SMF-Mg2+ approach for wastewater treatment.
Main Methods:
- Application of a weak static magnetic field (5 mT) coupled with Mg2+ stimulation.
- Monitoring granulation time, nutrient removal efficiency (COD, TN, PO43--P), and granule settleability (SVI30).
- Characterization of extracellular polymeric substances (EPS), microbial community structure, and surface interaction energetics using Extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.
Main Results:
- Granulation time reduced by 44.0% to 14 days.
- High removal efficiencies achieved: COD (95.1%), TN (78.6%), PO43--P (84.3%).
- Improved granule characteristics: average diameter (2.8 mm), settleability (SVI30 = 55.2 mL/g).
- SMF-Mg2+ coupling enhanced protein-dominated EPS, enriched functional bacteria (e.g., Thauera), and reduced surface energy barriers, promoting aggregation.
- Techno-economic assessment indicated a potential 59% reduction in operational expenditure (OPEX).
Conclusions:
- The synergistic SMF-Mg2+ strategy significantly accelerates ABGS granulation and enhances stability.
- This approach optimizes wastewater treatment performance, achieving high nutrient removal and excellent settleability.
- The physical-ionic stimulation offers a scalable and cost-effective solution to overcome ABGS start-up limitations, promoting sustainable wastewater management.
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