Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.

Nature methods·2026

Same author

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor.

ACS omega·2026

Same author

Absorption Mode Broadband 2D MS for Proteomics and Metabolomics.

Journal of the American Society for Mass Spectrometry·2026

Same author

Structural domain plasticity drives Zn(II) distribution and buffering in metallothionein-3.

Chemical communications (Cambridge, England)·2026

Same author

A Prototype timsOmni Platform Enables Confident Annotation of the Key Hypervariable CDR3 Regions of IgG Immunoglobulins Using Low- and High-Energy Electron-Based Fragmentation.

Analytical chemistry·2026

Same author

Propionic Acid Outperforms Formic and Acetic Acid in MS Sensitivity for High-Flow Reversed-Phase LC-MS Bottom-Up Proteomics.

Analytical chemistry·2026

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

An Amphibious Bifunctional Probe for Protein Chemical Cross-Linking.

Michael Karpíšek^1,2, Lukáš Fojtík^1,2, Jan Fiala^1,2

¹Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.

Journal of the American Chemical Society

|July 8, 2026

Summary

This summary is machine-generated.

New Togni cross-linking reagents (TR1 and TR2) enable efficient protein structure analysis using chemical cross-linking mass spectrometry (CXMS). These reagents offer versatile activation and target specific amino acids for improved protein interaction studies.

More Related Videos

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Related Experiment Videos

Last Updated: Jul 9, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Area of Science:

Biochemistry
Chemical Biology
Proteomics

Background:

Chemical cross-linking mass spectrometry (CXMS) is vital for studying protein structure, dynamics, and interactions.
Developing novel cross-linking reagents is crucial for enhancing CXMS capabilities and reliability.

Purpose of the Study:

To design and synthesize novel bis-hypervalent iodine reagents for protein cross-linking.
To evaluate the efficacy and versatility of these new reagents in CXMS applications.

Main Methods:

Synthesis of two novel bis-hypervalent iodine reagents, Togni cross-linking reagents TR1 and TR2.
Application of reagents for intra- and intermolecular cross-linking of diverse proteins (apomyoglobin, holomyoglobin, RHOA).
Utilized ascorbate or thiol-based redox activation and acyl fluoride formation for cross-linking.

Main Results:

TR1 and TR2 efficiently cross-linked proteins, targeting aromatic amino acids, cysteines, and lysines.
Demonstrated successful application in studying structurally diverse proteins under mild aqueous conditions.
Validated dual activation modes (ascorbate and redox) and bifunctional acylating capabilities.

Conclusions:

Togni cross-linking agents represent valuable additions to the CXMS toolbox.
Amphibious bis-hypervalent iodine-fluoroalkyl chemistry shows significant potential for protein structure and dynamics research.