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Related Experiment Video

Updated: Jul 9, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

Establishing MS2-MCP-based single-molecule RNA visualization in Schizosaccharomyces pombe.

Douglas E Weidemann1, Sarah C Turner1, Samir G Chethan1

  • 1Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.

Journal of Cell Science
|July 8, 2026
PubMed
Summary

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Chromosome segregation synchrony in S. pombe is noise limited and arises without positive feedback.

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Chromosome segregation synchrony in <i>S. pombe</i> is noise-limited and arises without positive feedback.

bioRxiv : the preprint server for biology·2026
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Establishing MS2-MCP-based single-molecule RNA visualization in <i>Schizosaccharomyces pombe</i>.

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A distinct phase of cyclin B (Cdc13) nuclear export at mitotic entry in <i>Schizosaccharomyces pombe</i>.

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A Comparative Study of Algorithms Detecting Differential Rhythmicity in Transcriptomic Data.

Bioinformatics and biology insights·2024

Researchers developed single-molecule RNA imaging for fission yeast using the MS2-MS2 bacteriophage system. This breakthrough enables quantitative analysis of RNA dynamics in this key model organism.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Single-molecule RNA imaging is crucial for understanding RNA biology.
  • The MS2-MCP system is widely used but not established in fission yeast (Schizosaccharomyces pombe).
  • Fission yeast is a vital model for eukaryotic gene expression studies.

Purpose of the Study:

  • To establish single-molecule RNA imaging in fission yeast.
  • To optimize the MS2-MCP system for S. pombe.
  • To enable quantitative analysis of RNA dynamics in fission yeast.

Main Methods:

  • Systematic optimization of MCP (MS2 coat protein) expression and localization.
  • Screening of constitutive S. pombe promoters.
  • Evaluation of nuclear localization and export signals (NLSs/NESs).
Keywords:
Fission yeastMS2–MCP systemRNA dynamicsSingle-molecule RNA imaging

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Last Updated: Jul 9, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Published on: June 14, 2013

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  • Utilizing tandem StayGold fluorescent tag for superior photostability.
  • Development of optimized vectors for MS2 stem-loop tagging of endogenous transcripts.

    • Main Results:

    • Successful implementation of single-molecule RNA imaging in S. pombe.
    • Optimized MCP expression and localization achieved.
    • Tandem StayGold tag demonstrated superior photostability.
    • Developed tools for tagging endogenous transcripts.

    Conclusions:

    • Single-molecule RNA imaging is now feasible in fission yeast.
    • The developed tools facilitate quantitative RNA dynamics studies.
    • This advancement opens new avenues for research in a core genetic model.