Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the learning curve for ultrasound-guided distal radial artery cannulation in novices using a cumulative sum analysis: A prospective observational study.

Medicine·2025
Same author

Secreted frizzled-related protein 1 regulates the progression of neuropathic pain in mice following spinal nerve ligation.

Journal of cellular physiology·2017
See all related articles

Related Experiment Video

Updated: Jul 9, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

Mendelian Randomization and Transcriptome Analysis Identify Ischemic Stroke Biomarkers With Putative Relevance to

Jingwei Xiong1, Jie Zhang1, Xuemei Cheng1

  • 1Department of Anesthesiology, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China, nju.edu.cn.

Biomed Research International
|July 8, 2026
PubMed
Summary

This study identifies CDH7, MGAT4C, and ITPKC as promising biomarkers for ischemic stroke (IS) by integrating genetic and gene expression data. These proteins show altered levels and potential diagnostic value for IS.

Keywords:
biomarkerscirculating proteinsischemic strokemendelian randomizationtranscriptome analysis

More Related Videos

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
09:48

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Published on: May 4, 2015

Related Experiment Videos

Last Updated: Jul 9, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
09:48

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Published on: May 4, 2015

Area of Science:

  • Biochemistry and Molecular Biology
  • Genetics and Genomics
  • Neuroscience

Background:

  • Circulating proteins are implicated in ischemic stroke (IS) pathogenesis, but effective biomarkers are underutilized.
  • This study leverages plasma protein genome-wide association study (GWAS) data and cerebrospinal fluid (CSF) relevance to identify novel IS biomarkers.

Purpose of the Study:

  • To identify and validate potential circulating protein biomarkers for ischemic stroke (IS).
  • To integrate Mendelian randomization (MR) and transcriptomic analyses for robust biomarker discovery.
  • To explore the functional mechanisms and therapeutic potential related to identified biomarkers.

Main Methods:

  • Two-sample Mendelian randomization (MR) analysis to assess genetic associations between circulating proteins and IS.
  • Transcriptomic analysis to identify differentially expressed genes (DEGs) in relevant datasets.
  • Functional enrichment, protein-protein interaction (PPI), and machine learning (LASSO/SVM-RFE) for candidate biomarker screening.
  • Gene set enrichment analysis (GSEA), immune infiltration, and drug prediction for mechanistic insights.
  • Validation using RT-qPCR and immunohistochemistry in a rat model of ischemic stroke (MCAO/R).

Main Results:

  • MR analysis revealed 157 protein-related genes with genetic links to IS.
  • Transcriptomics identified 4144 DEGs, with 46 overlapping with MR-derived genes (MRGs).
  • Machine learning prioritized CDH7, MGAT4C, and ITPKC as top candidates due to high diagnostic accuracy (AUC > 0.7) and differential expression.
  • CDH7 and MGAT4C showed high expression, while ITPKC showed low expression in an ischemic stroke rat model, consistent with computational predictions.

Conclusions:

  • CDH7, MGAT4C, and ITPKC are identified as promising biomarkers for ischemic stroke (IS).
  • These candidates demonstrate genetic association and transcriptional alterations relevant to IS.
  • Further investigation is warranted to explore their clinical utility and mechanistic roles in ischemic stroke.