Related Experiment Video

Updated: Jul 9, 2026

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

Inhibition of Colorectal Cancer Cell Progression by Picroside II Through Modulation of the Notch1 Signaling Pathway

Rui Wang1,2, Wei Zhang3, Fang Wang3

  • 1Department of Radiology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.

Journal of Biochemical and Molecular Toxicology
|July 8, 2026
PubMed

Insights

Picroside II effectively inhibits colorectal cancer (CRC) cell progression by suppressing proliferation, migration, and invasion. This compound acts by downregulating the Notch1 signaling pathway, offering a potential therapeutic strategy for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Colorectal cancer (CRC) remains a significant global health challenge.
  • Identifying novel therapeutic agents targeting cancer cell progression is crucial.
  • The Notch1 signaling pathway is implicated in various cancers, including CRC.

Purpose of the Study:

  • To investigate the anti-cancer effects of Picroside II on colorectal cancer (CRC) cells.
  • To determine the impact of Picroside II on CRC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • To explore the role of the Notch1 signaling pathway in mediating the effects of Picroside II.

Main Methods:

  • Human CRC cell lines (SW480, SW620) were treated with varying concentrations of Picroside II.
  • Cell proliferation was assessed using CCK-8, colony formation, and EdU assays.
  • Migration, invasion, EMT markers, and Notch1 pathway proteins were analyzed via wound healing, Transwell, and Western blotting assays.

Main Results:

  • Picroside II significantly inhibited CRC cell proliferation, colony formation, and DNA synthesis.
  • Treatment with Picroside II reduced CRC cell migration and invasion, altering EMT markers (upregulating E-cadherin, downregulating N-cadherin, Vimentin, ZEB2).
  • Picroside II decreased the expression of Notch1, Cleaved Notch1, RBP, and HES1 proteins in a dose-dependent manner.

Conclusions:

  • Picroside II exhibits potent anti-cancer properties against colorectal cancer cells in vitro.
  • The mechanism involves the inhibition of the Notch1 signaling pathway.
  • Picroside II represents a promising candidate for further investigation as a CRC therapeutic agent.
Keywords:
Notch1Picroside IIcolorectal cancerepithelial–mesenchymal transitionmigrationproliferation

More Related Videos

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine06:24

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Published on: October 13, 2023

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...