Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning hydrogel affinity to control the release of antibodies.

Biomaterials·2026
Same author

Toward biomimetic optogenetics: Drug-free activation of acute opiate reward.

iScience·2025
Same author

Pancreatic stem cells originate during the pancreatic progenitor developmental stage.

Frontiers in cell and developmental biology·2025
Same author

Neural crest precursors from the skin are the primary source of directly reprogrammed neurons.

Stem cell reports·2024
Same author

Constraint-Induced Movement Therapy (CIMT) and Neural Precursor Cell (NPC) Transplantation Synergistically Promote Anatomical and Functional Recovery in a Hypoxic-Ischemic Mouse Model.

International journal of molecular sciences·2024
Same author

Connexin-36-positive gap junctions in ventral tegmental area GABA neurons sustain opiate dependence.

The European journal of neuroscience·2024

Related Experiment Video

Updated: Jul 10, 2026

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
08:17

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Precision ventral extraction of implanted mouse brains: A protocol.

Lyla El-Fayomi1, Michael Bergamini2, Derek van der Kooy3

  • 1Krembil Research Institute, Toronto Western Hospital, Toronto, ON M5T 0S8, Canada.

STAR Protocols
|July 8, 2026
PubMed
Summary

This study presents a refined protocol for extracting intact mouse brains with neural implants, minimizing tissue damage during histology. The method ensures reproducible results for neuroscience research.

Keywords:
Biotechnology and bioengineeringHealth SciencesModel OrganismsNeuroscience

More Related Videos

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
07:49

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Published on: June 2, 2022

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
11:11

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Published on: October 8, 2021

Related Experiment Videos

Last Updated: Jul 10, 2026

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
08:17

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
07:49

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Published on: June 2, 2022

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
11:11

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Published on: October 8, 2021

Area of Science:

  • Neuroscience
  • Histology
  • Surgical Techniques

Background:

  • Neural implants are essential for mouse studies but complicate brain extraction for histology.
  • Permanent implants often lead to tissue distortion and damage during brain removal.

Purpose of the Study:

  • To provide a detailed protocol for minimizing trauma during mouse brain extraction with neural implants.
  • To ensure reproducible and accurate histological analysis of brain tissue.

Main Methods:

  • A precise dissection technique for cranial isolation and brain extraction from the ventral aspect.
  • Detailed steps for transcardial perfusion, post-fixation, and cryoprotection.
  • Utilizing clear anatomical landmarks for consistent execution.

Main Results:

  • Successful extraction of intact mouse brains with minimal tissue trauma and distortion.
  • Demonstrated reproducibility of the brain extraction protocol.
  • Facilitated clean separation of the brain from the skull.

Conclusions:

  • The described protocol offers a reliable method for extracting neural-implanted mouse brains for histology.
  • This technique enhances the quality of tissue for subsequent analyses.
  • It addresses common challenges in preparing neural-implanted samples for research.