Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alveoli and Alveolar Ducts01:26

Alveoli and Alveolar Ducts

The respiratory zone of the human body, which stands in contrast to the conducting zone, comprises the structures that actively participate in the exchange of gases. The initiation of this zone is marked by the terminal bronchioles converging into respiratory bronchioles, the tiniest bronchiole classification. The respiratory bronchioles give way to the alveolar ducts that opens into a congregation of alveoli. Actively involved in gas exchange, alveoli resemble tiny sacs similar to clusters of...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circulating metabolites and fatty acids associated with ultra-processed food consumption: results from the EPIC study.

Critical reviews in food science and nutrition·2026
Same author

Ubiquitin Ligase COP1 Suppresses Neuroinflammation by Degrading c/EBPβ in Microglia.

Cell·2026
Same author

Time Is Kidney: A Case Study and Literature Review of Bilateral Renal Compartment Syndrome After Blunt Trauma, a Rare Complication.

Journal of clinical medicine·2026
Same author

Proteomics signature of moderate-to-vigorous physical activity and risk of multimorbidity of cancer and cardiometabolic diseases.

Communications medicine·2026
Same author

VSIG4 Restricts Hepatocellular Carcinoma Control by Suppressing Tumor-Specific CD8+ T-cell Immunity in the Tumor Microenvironment.

Cancer immunology research·2026
Same author

Convergent genomic and molecular features predict risk of metachronous metastasis in clear cell renal cell carcinoma.

Communications medicine·2026

Related Experiment Video

Updated: Jul 10, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
11:13

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Mapping the dialogue: Decoding alveolar stem-niche interactions.

Ahmad N Nabhan1,2, Anne Biton3, Christine Everett4

  • 1Department of Molecular and Cellular Biology, University of California, Berkeley, CA 94720.

Proceedings of the National Academy of Sciences of the United States of America
|July 8, 2026
PubMed
Summary
This summary is machine-generated.

Lung stem cells significantly influence fibroblast behavior, impacting tissue repair and immune responses. Stem cell identity, particularly Nkx2.1 expression, is crucial for these interactions, revealing new insights into lung tissue regulation.

Keywords:
Nkx2.1epitheliumfibroblastlungniche

More Related Videos

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement
05:25

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Published on: July 21, 2023

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Related Experiment Videos

Last Updated: Jul 10, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
11:13

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement
05:25

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Published on: July 21, 2023

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Area of Science:

  • Lung biology and regenerative medicine.
  • Cellular interactions and tissue engineering.

Background:

  • Cellular atlases show diverse cell phenotypes, but how they interact to affect tissue behavior is unclear.
  • Understanding lung stem cell-niche interactions is vital for tissue repair and disease modeling.

Purpose of the Study:

  • To develop an alveolosphere screening platform to investigate lung stem cell-fibroblast niche interactions.
  • To map gene functions in stem cells and their impact on the niche.
  • To uncover cellular states and pathways governing stem cell-niche communication.

Main Methods:

  • Alveolosphere screening platform for high-throughput gene function assessment in lung stem cells.
  • Imaging-based assessment of 201 candidate genes in stem cells.
  • Chimeric RNA sequencing (RNAseq) for cell-autonomous and non-cell-autonomous effects analysis.

Main Results:

  • A phenome-transcriptome map revealed pathways regulating proliferation, metabolism, and immune signaling in stem cells and their niche.
  • Lung stem cells were found to influence fibroblast scar-forming and immune programs.
  • Loss of Nkx2.1 (lung epithelial identity transcription factor) significantly altered stem cell-niche interactions and non-cell-autonomous effects.

Conclusions:

  • Functional atlases provide insights into cellular interactions complementing descriptive atlases.
  • Stem cell identity plays a critical role in modulating niche responses, including injury and immune signaling.
  • Nkx2.1 is a key regulator of lung stem cell-niche communication and non-cell-autonomous effects.