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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
An engineering strategy from biomass support to a precision-recognition interface: amino acid-assisted imprinted
Xinya Xu1, Yanhui Ge2, Yundong Xie3
1College of Pharmacy, Shaanxi University of Chinese Medicine, Xi'an 712046, China; Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine, Xianyang 712046, China.
None:
An integrated strategy was developed to fabricate papain-imprinted polymers using magnetic biochar derived from waste tangerine peel. The biochar surface was modified with amino acids to improve protein affinity, and a biocompatible imprinting layer was subsequently formed through the mild polymerization of dual functional monomers. The optimized imprinted polymers exhibited high adsorption capacity (197.31 mg/g), strong selectivity (IF = 6.12), and robust reusability. Their utility was demonstrated through one-step selective purification of papain from complex samples, including crude commercial enzyme and spiked biological matrices. This work provides a sustainable strategy that combines biomass valorization with molecular imprinting for the purification of high-value enzymes in the food and biopharmaceutical industries.
