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Updated: Jul 10, 2026
10:01
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Published on: November 28, 2017
Peptide crosslinking by cytochrome P450 enzymes: Diversity, discovery and developments
Lauren A M Murray1, Alicia M Kirk2, James J De Voss3
1Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
Current Opinion in Chemical Biology
|July 8, 2026
Summary
Cytochrome P450 enzymes catalyze peptide cyclization reactions. These versatile enzymes show potential for engineered biocatalysis in chemoenzymatic synthesis and altering biosynthesis pathways.
Area of Science:
- Biochemistry
- Enzymology
- Synthetic Biology
Background:
- Cytochrome P450s are crucial oxidative enzymes.
- They perform diverse oxidation reactions across biological systems.
- Their role in peptide modification is an emerging area of research.
Purpose of the Study:
- To summarize recent findings on the role of Cytochrome P450s in peptide cyclization.
- To highlight the potential of P450s as biocatalysts.
- To explore their application in chemoenzymatic synthesis and metabolic engineering.
Main Methods:
- Literature review of recent studies on P450-mediated peptide cyclization.
- Analysis of P450 enzyme mechanisms in cyclization reactions.
- Discussion of engineering strategies for P450 biocatalysts.
Main Results:
- Cytochrome P450 enzymes are widely involved in the cyclization of peptides.
- These enzymes demonstrate significant versatility in catalyzing complex reactions.
- Evidence suggests P450s can be engineered for specific synthetic applications.
Conclusions:
- Cytochrome P450s play a significant role in natural peptide cyclization.
- Engineered P450s offer promising biocatalytic solutions for chemoenzymatic synthesis.
- P450s can be utilized to modify biosynthesis pathways in vivo.
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