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Assays for the Identification of Novel Antivirals against Bluetongue Virus
Published on: October 11, 2013
Discovery of broad-spectrum antiviral activity in Amaryllidaceae alkaloids
Yuna Aoki1, Koji Onomoto1, Ka-Ho Kato1
1Division of Molecular Immunology, Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, 260-8673, Japan.
Abstract:
RNA virus infections pose a serious threat to public health, underscoring the need for broad-spectrum antiviral agents. In this study, we screened 383 plant-derived natural products with distinctive and structurally unique frameworks. We identified three Amaryllidaceae alkaloids, haemanthamine, crinamine, and 6-hydroxycrinamine, that exhibited inhibitory activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero E6/TMPRSS2 cells. Notably, these compounds concentration-dependently inhibited the replication of not only SARS-CoV-2 but also encephalomyocarditis virus, Sindbis virus, vesicular stomatitis virus, and influenza A virus, with antiviral activity distinguishable from cytotoxicity. These results suggest that Amaryllidaceae alkaloids possess broad-spectrum antiviral activity and are promising candidates for future antiviral drug development.
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