Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems

Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug absorption...

Measurement of Bioavailability: Pharmacodynamic Methods

Measurement of Bioavailability: Pharmacodynamic Methods

Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Generic intravenous (IV) drugs are considered bioequivalent to their branded counterparts due to their 100% bioavailability upon administration. However, variations in stability among different drug products can significantly influence their therapeutic performance, even if they are pharmaceutically equivalent.Cefuroxime, a prophylactic antimicrobial, is often used as a single-dose IV injection for patients undergoing coronary artery bypass grafting surgery. A 3 g dose typically provides...

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast, controlled...

Impact of Pharmacokinetic–Pharmacodynamic Models: Regulatory Decisions

Impact of Pharmacokinetic–Pharmacodynamic Models: Regulatory Decisions

PK–PD modeling has significantly influenced FDA regulatory decisions, particularly drug approval, dosage optimization, and labeling. These models integrate pharmacokinetics (PK) and pharmacodynamics (PD) to predict drug behavior and effects, aiding in optimizing dosing regimens and enhancing the probability of clinical trial success.One notable example is Nesiritide (Natrecor®), a recombinant human brain natriuretic peptide for treating acute decompensated congestive heart failure (CHF).

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrasmall surface functionalized nanoclusters (Ni, Cu and Co) for high performance oxygen evolution catalysis.

RSC advances·2026

Same author

CircRNAs as central regulators of Immunometabolic crosstalk in hepatocellular carcinoma.

Clinica chimica acta; international journal of clinical chemistry·2026

Same author

Critical Evaluation of Analytical Strategies for the Quantification of Procaine and Its Metabolites Across Diverse Matrices.

Critical reviews in analytical chemistry·2026

Same author

Comprehensive Review of Analytical Methods for the Determination of Lomefloxacin Across Multiple Matrices.

Critical reviews in analytical chemistry·2026

Same author

Synthetic advances, pharmacological prospects, and molecular insights of spirooxindoles: a comprehensive review.

Journal of molecular modeling·2026

Same author

Novel nano-catalytic strategy for selective imine-chalcone hybrid functionalization: synthesizing, bioactivity evaluations, molecular docking, ADME, DFT, and SAR analysis.

Bioorganic chemistry·2026

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Chemical Cartography Approaches to Study Trypanosomatid Infection

Chemical Cartography Approaches to Study Trypanosomatid Infection

Published on: January 21, 2022

Matrix Effects and Analytical Instability in Pharmaceutical Bioanalysis: Current Challenges and Future Directions.

Hemn A H Barzani¹, Rebaz Anwar Omer², Nergz Bayiz Abdulrahman³

¹Department of Medical Laboratory Science, College of Health Science, Lebanese French University, Erbil, Iraq.

Critical Reviews in Analytical Chemistry

|July 9, 2026

Summary

Pharmaceutical bioanalysis faces challenges from complex matrices and analyte instability. Intelligent analytical technologies and advanced sample preparation enhance accuracy and reproducibility for drug development and diagnostics.

Keywords:

Artificial intelligence in analytical chemistry LC–MS/MS analytical instability matrix effects pharmaceutical bioanalysis

More Related Videos

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

Related Experiment Videos

Last Updated: Jul 10, 2026

Chemical Cartography Approaches to Study Trypanosomatid Infection

Chemical Cartography Approaches to Study Trypanosomatid Infection

Published on: January 21, 2022

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

Area of Science:

Pharmaceutical bioanalysis
Analytical chemistry
Drug development

Background:

Complex biological and pharmaceutical matrices cause matrix effects (ion suppression/enhancement) and analyte instability.
These issues compromise sensitivity, accuracy, and reproducibility in drug development and diagnostics.
Challenges include interference from endogenous compounds and degradation during sample handling.

Purpose of the Study:

To review the mechanistic basis of matrix effects and analytical instability in pharmaceutical bioanalysis.
To highlight advancements in intelligent analytical technologies for robust and sustainable bioanalysis.
To discuss the future of automated and smart analytical ecosystems in pharmaceutical analysis.

Main Methods:

Critical evaluation of matrix effects and analyte instability mechanisms.
Review of advanced sample preparation techniques (SPE, phospholipid removal, MIP, microextraction).
Assessment of modern LC-MS platforms (UHPLC-MS/MS, HRMS) and emerging technologies (AI, microfluidics, biosensors).

Main Results:

Advanced sample preparation and LC-MS platforms significantly improve trace-level analysis in complex matrices.
Intelligent technologies like AI, microfluidics, and biosensors are creating automated analytical ecosystems.
These innovations enhance analytical robustness, reproducibility, and sustainability.

Conclusions:

Future pharmaceutical bioanalysis will integrate AI, advanced LC-MS, green chemistry, and regulatory harmonization.
The goal is to improve clinical applicability, analytical reliability, and environmental sustainability.
Intelligent, automated, and sustainable systems are key to advancing the field.