Related Experiment Video
Updated: Jul 10, 2026

04:40
Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth
Published on: March 27, 2026
Effect of Dehydration Time on Color Difference and Recovery After Tooth Fragment Reattachment: An In Vitro Study.
Daniella Birnbaum Pessoa De Mello1, Nathally Lauise Santos Sousa1, Liliana Vicente Melo De Lucas Rezende1
1University Hospital of Brasilia, University of Brasilia, Brasilia, Brazil.
Summary
Fragment dehydration significantly impacts the long-term color stability of reattached dental fragments, leading to noticeable esthetic changes. Other factors also contribute to color alterations over time.
Area of Science:
- Dental materials science
- Esthetic dentistry
- Biomaterials
Background:
- Reattaching dental fragments is a conservative treatment for enamel-dentin fractures.
- Fragment dehydration can negatively affect the esthetic outcome of reattached teeth.
- Long-term color stability is crucial for successful dental fragment reattachment.
Purpose of the Study:
- To evaluate the influence of different fragment dehydration periods on the color differences of reattached dental fragments.
- To assess the long-term color stability of reattached fragments over a one-year period.
- To compare color changes in dehydrated versus non-dehydrated reattached fragments.
Main Methods:
- 48 bovine incisors with simulated enamel-dentin fractures were divided into four groups based on dehydration time (0, 12, 24, 48 hours).
- Fragments were reattached using a universal adhesive and bulk-fill resin composite after a 20-minute rehydration period.
- Color measurements were taken at baseline and multiple time points up to one year using CIELAB and CIEDE2000 systems.
Main Results:
- Significant interactions were found between dehydration time, evaluation period, and measurement location for color differences.
- Dehydrated fragments generally showed greater color differences compared to the control group, especially in the fragment area.
- Color differences persisted throughout the year, with no complete return to baseline values; even the control group exhibited significant color changes.
Conclusions:
- Fragment dehydration significantly influences the long-term color behavior of reattached dental fragments, causing clinically perceptible differences.
- Factors beyond dehydration contribute to chromatic alterations in reattached fragments over time.
- The study highlights the importance of considering dehydration effects and other variables for predictable esthetic outcomes in dental fragment reattachment.
