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Yinhuapinggan granules regulate PI3K/AKT and RAS/MAPK pathways to inhibit Streptococcus Pneumoniae-induced
Chujun Gu1,2, Daojun Yu3, Tianhang Chen4,2
1College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China.
Archives of Microbiology
|July 9, 2026
Summary
Yinhuapinggan Granules (YHPG) effectively treat Streptococcus pneumoniae infections in mice by reducing lung damage and inflammation. YHPG works by modulating the PI3K/AKT and RAS/MAPK signaling pathways, offering a potential new treatment for drug-resistant pneumonia.
Area of Science:
- Microbiology
- Pharmacology
- Immunology
Background:
- Streptococcus pneumoniae causes pneumonia, with drug-resistant strains posing a significant challenge.
- Traditional Chinese Medicine (TCM) shows potential for treating resistant infections, but mechanisms are unclear.
- Yinhuapinggan Granules (YHPG) are a TCM formulation requiring mechanistic investigation.
Purpose of the Study:
- To investigate the efficacy of Yinhuapinggan Granules (YHPG) against Streptococcus pneumoniae infection in a mouse model.
- To elucidate the underlying mechanism of YHPG action, focusing on PI3K/AKT and RAS/MAPK pathways.
Main Methods:
- Established a mouse model of Streptococcus pneumoniae pneumonia via intranasal instillation.
- Administered varying doses of YHPG post-infection for six days.
- Utilized Hematoxylin-eosin staining, immunohistochemistry, qPCR, and Western blotting to assess lung pathology, inflammation, and signaling pathway protein expression.
Main Results:
- YHPG significantly reduced lung damage, inflammation, and neutrophil infiltration in infected mice.
- YHPG inhibited the upregulation of key signaling proteins (IκBα, JNK, P38, ERK) induced by S. pneumoniae.
- These effects were linked to the modulation of PI3K/AKT and RAS/MAPK signaling pathways.
Conclusions:
- Yinhuapinggan Granules (YHPG) demonstrate therapeutic efficacy against Streptococcus pneumoniae pneumonia in mice.
- YHPG exerts anti-inflammatory and immunomodulatory effects by regulating PI3K/AKT and RAS/MAPK pathways.
- YHPG represents a promising therapeutic agent for drug-resistant bacterial pneumonia.
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