Related Experiment Video
Updated: Jul 12, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
Reactive oxygen species in cumulus cells inhibit the acrosome reaction in human sperm
Ana Laura Gutiérrez Silva1, Jorge Arturo Torres Juárez2, Haydé Azeneth Vergara Castañeda2
1Unidad de Investigación Genética, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, México.
Abstract:
The present work aimed to analyze the effect of reactive oxygen species in cumulus cells on the acrosome reaction of human spermatozoa. For this purpose, we first evaluated the effect of reactive oxygen species in human sperm on the acrosome reaction, and the effect of reactive oxygen species in cumulus cells alone. Finally, we co-incubated human spermatozoa with human cumulus cells, previously exposed to different concentrations of hydrogen peroxide, and measured the percentage of reacting spermatozoa. Our results show an interaction effect between the levels of reactive oxygen species in human sperm and their capacitation state. High levels of reactive oxygen species induced the acrosome reaction in non-capacitated sperm, while reducing the percentage of acrosome reaction in capacitated sperm. Furthermore, our results show that, while non-stressed cumulus cells are capable of inducing the acrosome reaction, high levels of reactive oxygen species in cumulus cells hinder this capacity. These results suggest that, in the conditions used in this study, an oxidative environment in cumulus cells limits the number of sperm that react.
More Related Videos
05:44Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
Published on: March 1, 2019
08:06The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization
Published on: October 18, 2024
Related Concept Videos
Fertilization
Sperm Structure and Semen Composition
Spermatogenesis
Sperm Transport
The maturation phase occurs in the epididymis, where sperm...
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Radical Autoxidation